Waveform pulses
Références:
M Golzio, MP Rols and J Teissié. In vitro and in vivo electric field-mediated permeabilization, gene transfer, and expression. Methods 33(2004):126-135
Paganin-Gioanni A, Bellard E, Couderc B, Teissié J, Golzio M. Tracking in vitro and in vivo siRNAelectrotransfer in tumor cells. J RNAi Gene Silencing. 2008 May 27;4(1):281-8.
Rosazza C, Escoffre JM, Zumbusch A, Rols MP. The actin cytoskeleton has an active role in the electrotransfer of plasmid DNA in mammalian cells. MolTher. 2011 May;19(5):913-21.
Escoffre JM, Portet T, Favard C, Teissié J, Dean DS, Rols MP. Electromediated formation of DNA complexes with cell membranes and its consequences for gene delivery.BiochimBiophysActa. 2011 Jun;1808(6):1538-43.
Paganin-Gioanni A, Bellard E, Escoffre JM, Rols MP, Teissié J, Golzio M. Direct visualization at the single-cell level of siRNAelectrotransfer into cancer cells.ProcNatlAcadSci U S A. 2011 Jun 28;108(26):10443-7.
Markelc B, Tevz G, Cemazar M, Kranjc S, Lavrencak J, Zegura B, Teissie J, Sersa G. Muscle gene electrotransfer is increased by the antioxidant tempol in mice.. Gene Ther. 2012 Mar;19(3):312-20
S Chabot, J Orio, M Schmeer, M Schleef, M Golzio and J Teissié.Minicircle DNA electrotransfer for efficient tissue-targeted gene delivery. Gene Therapy (2013) 20,62-68
ELECTRO cell S20
BIO-MEDICAL RESEARCH > ELECTRO cell S20
ELECTRO cell S20 is an electroporation device used to enter the DNA into cells by the application of high current pulses to the mixture in suspension.
This is a Pulse Generator High Voltage Unipolar.
ELECTRO S20 cell consists of:
- A high-voltage generator supplying the electric pulses
- An integrated pulse control software
- An intuitive LCD touch screen and easy to use
- An electrode holder provided with a trigger switch pulses compatible with all the electrodes of commerce
- A pair of electrodes
- A foot pedal controls impulses.
The electroporator has an internal capacitor that is charged to a programmable voltage in a range between 0 and 2000V. Once the charged capacitor, it is able to send a predetermined number of pulses by controlling the pulse width and period.
The parameters U, T, P and R are configurable.
- U (pulse amplitude): 0V - 2 000V.
- T (pulse width) : 5μs - 50 000μs.
- P (repetition period of the pulse): 1ms - 000ms 10.
- R (number of repetitions of the pulse): 0 - 10 000.
If R is set in the range 1-10 000, the number of pulses to be sent corresponds to the value of R.
If R = 0, the pulses will be sent continuously regardless of the number sent.
Operating modes: Manual, External and Remote.
-mode Manual: The unit is controlled exclusively from the LCD interface it has. The parameters of the pulse can be configured, then sending using the screen touchscreen enabled.
External -mode: similar to Manual mode, the device configuration is performed from the LCD interface, but the pulses are only activated by the external pedal.
-mode Remote: used to control the device only from an external PC connected to the Ethernet port.
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